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在分子生物学实验技术体系中 ,感受态细胞的制备和转化是基本的实验操作。目前 ,氯化钙法感受态细胞的制备和转化 ,由于相对简便和重复性好 ,得到广泛地应用 ,但该方法还不够快捷。一步法感受态细胞的制备和转化虽然快捷 ,但所用的PEG335 0和DMSO远非CaCl2 那样廉价
In molecular biology experimental technology system, the preparation and transformation of competent cells is the basic experimental operation. At present, the preparation and transformation of competent cells of calcium chloride method are widely used because of its relative simplicity and repeatability, but the method is not fast enough. One-Step Competent Cell Preparation and Transformation Although fast, PEG335 0 and DMSO are far less expensive than CaCl2