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为了获得优良的菌株资源,采用溶磷圈法、钼锑抗比色法和Spot、S2比色法,定性、定量测定了分离自三叶草根际10株溶磷细菌的溶磷和分泌植物生长激素(IAA)的能力,结果表明:各菌株在PKO无机磷培养液中的有效磷增量在106.63～666.01mg/L(P<0.05),菌株ls1-3有效磷增量最大;各菌株在蒙金娜有机磷培养液中的有效磷增量在0.32～58.42mg/L(P<0.05),菌株lhs11有效磷增量最大;有些菌株既能溶解无机磷,又能溶解有机磷,如菌株lhs4和lhs11;有些菌株能溶解无机磷,但无溶解有机磷的能力,如菌株ls1-3和ls1-5。除3个菌株(ls2-11、ls2-16和ls3-2)外,其他菌株都能分泌IAA,分泌量在0.36～20.39mg/L(P<0.05),菌株ls3-5分泌能力最强(20.39μg/mL)。菌株ls1-3、ls2-3、ls3-5、lhs4和lhs11有望作为研制微生物肥料的优良菌株。
In order to obtain excellent strain resources, phosphate solubilizing bacteria, molybdenum-antimony colorimetric method and Spot, S2 colorimetric method were used to qualitatively and quantitatively determine the dissolved phosphorus and plant growth hormone secreted by 10 phosphate-solubilizing bacteria in the rhizosphere of clover (IAA). The results showed that the available phosphorus increment of each strain was between 106.63 and 666.01 mg / L (P <0.05) in PKO inorganic phosphorus medium, and the increment of available phosphorus in strain ls1-3 was the highest. The available phosphorus increment in Jinna organophosphorus broth ranged from 0.32 to 58.42mg / L (P <0.05), and the increment of available phosphorus in strain lhs11 was the highest. Some strains could dissolve both inorganic phosphorus and organic phosphorus, such as strain lhs4 And lhs11; some strains can dissolve inorganic phosphorus, but no ability to dissolve organic phosphorus, such as strains ls1-3 and ls1-5. Except for 3 strains (ls2-11, ls2-16 and ls3-2), all the other strains could secrete IAA with the secretion of 0.36 ~ 20.39mg / L (P <0.05) 20.39 μg / mL). Strain ls1-3, ls2-3, ls3-5, lhs4 and lhs11 are expected to be fine strains for the development of microbial fertilizers.