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作者应用ＣＤ系列单克隆抗体、碱性磷酸酶抗碱性磷酸酶双桥酶法（ＡＰＡＡＰ技术）对甲型肝炎患者５２例外周血Ｔ淋巴细胞亚群的变化进行了检测及分析。发现患者急性期ＣＤ４＋细胞百分率（３０．７±３．４％）较正常对照（３７．７±２．５％）明显降低（Ｐ＜０．０１），ＣＤ８＋率则显著增高（３５．８±３．９％对２７．７±２．４％，Ｐ＜０．０１），导致ＣＤ４＋／ＣＤ８＋比值（０．８７±０．２对１．３±０．１）明显下降（Ｐ＜０．０１）且倒置。恢复期则以上变化基本恢复正常。对其中１１例典型患者进行了为期１０周的动态观察，发现丙氨酸氨基转移酶（ＡＬＴ与ＣＤ８＋细胞百分率之间存在着非常显著的正相关（ｒ＝０．９６，Ｐ＜０．０１），而与ＣＤ４＋率及Ｃ０４＋／ＣＤ８＋之间均存在显著的负相关（ｒ＝－０．９６，Ｐ＜０．０１；ｒ＝－０．９５，Ｐ＜０．０１）。以上结果在一定程度上说明了细胞免疫可能参与了甲型肝炎的发病机理。
The authors used CD series of monoclonal antibodies, alkaline phosphatase anti-alkaline phosphatase double-bridge enzyme method (APAAP technology) in 52 cases of hepatitis A patients with peripheral blood T lymphocyte subsets were detected and analyzed. The percentage of CD4 + cells in the acute stage was significantly lower than that in the control group (30.7 ± 3.4% vs 37.7 ± 2.5%, P <0.01), and the percentage of CD8 + was significantly increased (35.8 ± 3.9% versus 27.7 ± 2.4%, P <0.01). The ratio of CD4 + / CD8 + was significantly decreased from 0.87 ± 0.2 to 1.3 ± 0.1 (P <0. 01) and inverted. During the recovery period, the above changes basically returned to normal. A typical 10-week dynamic observation of 11 patients showed that there was a significant positive correlation between the percentage of ALT and CD8 + cells (r = 0.96, P <0.01) (R = -0.96, P <0.01; r = -0.95, P <0.01) .There was a significant negative correlation between CD4 + and C04 + / CD8 + On the cellular immunity may be involved in the pathogenesis of hepatitis.