论文部分内容阅读
通过反复回交——筛选目的基因的手段,将C57BL/6-Tg(UBC-GFP)30Scha/J转基因小鼠的绿色荧光蛋白基因(GFP)分别向BABL/C、CBA/J、DBA/2J、AKR/J、FVB/J等品系小鼠导入,得到了5种不同遗传背景且带有GFP基因的导入系小鼠.冰冻组织切片等方法显示GFP导入系小鼠的皮肤、肝、肾、心、肺等均有不同程度的绿色荧光蛋白表达,不同遗传背景对GFP的组织表达谱、血液生理指标及繁殖性能未见影响.本实验采用基因导入法培育了5种新的带有GFP导入系小鼠,这些不同遗传背景的基因导入系小鼠为移植生物学研究提供了材料.
The GFP of C57BL / 6-Tg (UBC-GFP) 30Scha / J transgenic mice were transfected into BABL / C, CBA / J and DBA / 2J cells by repeated backcrossing-screening of target genes. , AKR / J, FVB / J and other strains of mice were introduced into five different genetic backgrounds with GFP gene-introduced mice.Freeze tissue sections and other methods to show GFP-derived mouse skin, liver, kidney, Heart and lung have different levels of green fluorescent protein expression, different genetic backgrounds of GFP tissue expression profile, blood physiological indicators and reproductive performance had no effect.In this experiment, gene introduction method to cultivate five new with GFP In mice, the introduction of genes from these different genetic backgrounds in mice provides materials for transplantation biology.