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应用酶联免疫吸附 ( ELISA)双抗体夹心法 ,研究了稻田 1 9种常见捕食性天敌对中性昆虫摇蚊的捕食作用。用摇蚊作抗原免疫雄性大白兔获得抗血清 ,用中和法、双向琼脂扩散实验及交叉反应对所制备的抗血清作特异性检测 ,抗体反应表明制备的抗血清对摇蚊抗原具较高的特异性。测定了 1 9种捕食者捕食摇蚊的临界吸收值。在检测的 1 9种捕食者中 ,有 1 3种捕食了摇蚊 ,占被检测捕食者种数的 6 7.89%。ELISA阳性反应率最高的是在早稻前期采集的褶管巢蛛 ,阳性率高达 5 0 % ,其次是晚稻中期采集的拟水狼蛛 ,其阳性率为 4 0 %。ELISA方法敏感 ,能快速检测捕食者对猎物的捕食作用及确定节肢类捕食者如蜘蛛对水稻害虫控制作用大小 ,作为一种有效实验工具 ,可有助于发展水稻害虫综合管理理论
The predation of nine common predatory natural enemies on Chilo suppressalis in paddy fields was studied by enzyme-linked immunosorbent assay (ELISA) sandwich enzyme-linked immunosorbent assay (ELISA). The antiserum was obtained by immunizing male rabbits with Chironomid as antigen and the specific antisera were detected by neutralization method, two-way agar diffusion assay and cross-reaction. The antibody reaction showed that the prepared antiserum had a higher Specificity. The critical uptake value of 19 species of predators was determined. Of the 19 species of predators tested, 13 species prey on the chironomid, accounting for 6 7.89% of the species tested. The highest positive ELISA positive rate was the Pleurotus nematode collected in the early stage of early rice, the positive rate was as high as 50%, followed by the mid-late rice collected Pirata subpiraticus, the positive rate was 40%. ELISA method is sensitive to rapid detection of predators prey on prey and determine the arthropod predators such as spiders on pest control the size of rice, as an effective experimental tool that can contribute to the development of rice pest integrated management theory