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目的：讨论肿瘤的浸润方式及 Ａｇ Ｎ Ｏ Ｒｓ 染色和 Ｐ５３ 蛋白表达在临床中的应用。方法：应用 Ｐｌｏｔｏｎ 一步法 Ａｇ Ｎ Ｏ Ｒｓ 染色和 Ｐ５３ 蛋白免疫组化方法观察 １９ 例喉癌、癌旁和相对正常粘膜中 Ｎ Ｏ Ｒｓ 的颗粒计数（ Ｇ Ｃ）、颗粒面积（ Ｇ Ｓ）和 Ｐ５３蛋白表达。结果：喉癌的 Ｇ Ｃ 和 Ｇ Ｓ最高，癌旁次之，相对正常粘膜最低，癌旁的 Ｇ Ｃ 和 Ｇ Ｓ有 ５ 例在均值以上。 Ｐ５３蛋白在 １９ 例喉癌中有 １４ 例呈阳性，在癌旁组织中有 ７ 例呈阳性。结论：喉癌的发展是多灶性和跳跃性的。手术的安全缘应该用 Ａｇ Ｎ Ｏ Ｒｓ染色和 Ｐ５３蛋白免疫组化方法确定。
Objective: To discuss the infiltration of tumor and Ag N O Rs staining and P53 protein expression in clinical application. Methods: The particle count (G C), particle area (G S) and P53 of N O Rs in 19 cases of laryngeal carcinoma, paracancer and normal mucosa were observed by Ploton one-step Ag N O Rs staining and P53 protein immunohistochemistry Protein. Results: The laryngeal carcinoma had the highest G C and G S, followed by the carcinoma, the lowest relative normal mucosa, and 5 cases of G C and G S adjacent to the cancer were above the mean. P53 protein in 14 cases of laryngeal cancer was positive in 14 cases, adjacent tissues in 7 cases were positive. Conclusion: The development of laryngeal cancer is multifocal and leapfrog. The safety margin of the operation should be confirmed by Ag N O Rs staining and P53 protein immunohistochemistry.