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目的:探讨血管内皮生长因子(VEGF)在高原脑水肿形成中的作用。方法:建立大鼠模拟高原模型,应用脑干湿重比率法定量脑水肿情况、应用荧光素钠透过率测定BBB通透性、应用实时荧光定量RT-PCR法检测脑组织VEGF mRNA含量以及应用蛋白印迹法半定量脑组织VEGF含量。结果:大鼠在高原24 h后脑组织含水率明显增高(P<0.05),荧光素钠透过率显著增加(P<0.01);VEGF mRNA转录及其表达显著增高(P<0.001)。结论:VEGF表达在高原脑水肿形成中起重要作用。
Objective: To investigate the role of vascular endothelial growth factor (VEGF) in the formation of cerebral edema. Methods: The rat model of plateau hyperalgesia was established. The brain stem wet weight ratio was used to quantify brain edema. The permeability of BBB was measured by fluorescein sodium transmittance. The content of VEGF mRNA in brain tissue was detected by real-time fluorescence quantitative RT-PCR. Western blotting semi-quantitative VEGF content in brain tissue. Results: After 24 hours, the water content of brain tissue in rats increased significantly (P <0.05) and the permeability of sodium fluorescein increased significantly (P <0.01). The transcription and expression of VEGF mRNA increased significantly (P <0.001). Conclusion: VEGF expression plays an important role in the formation of cerebral edema at high altitude.