运用改造后的乙型肝炎病毒表面抗原Ｓ＋ＰｒｅＳ１融合基因（将ＰｒｅＳ１区肝细胞受体结合位点（２１－４７ａａ）编码基因融合到Ｓ蛋白Ｃ端２２３位残基下游），构建了一系列真核表达载体，并在ＣＨＯ细胞上有效表达了分泌型的、具有Ｓ＋ＰｒｅＳ１双重抗原性的融合蛋白颗粒。用上述质粒ＤＮＡ肌肉免疫Ｃ５７ＢＬ／６Ｊ小鼠，成功地检测到了抗－ＨＢｓ和抗－ＰｒｅＳ１抗体，加强免疫能显著改善免疫效果。本研究还建立了检测抗－ＰｒｅＳ１的竞争抑制法和羊抗人ＨＢｓＡｇ封闭法，两种方法符合率达９６％，但后者更为敏感。用羊抗人ＨＢｓＡｇ封闭法测得抗－ＰｒｅＳ１滴度最高可达１：１２８０以上。 Using a reconstructed S + PreS1 fusion gene of hepatitis B virus surface (encoding the gene encoding PreS1 hepatocyte receptor binding site (21-47aa) to the downstream of residue 223 of C-terminal of S protein), a series of eukaryotic Expression vector and efficiently expressed secreted, fusion protein particles with S + PreS1 dual antigenicity on CHO cells. Immunization of C57BL / 6J mice with the above plasmid DNA successfully detected anti-HBs and anti-PreS1 antibodies, and boosting immunity significantly improved the immune response. In this study, we also established competitive inhibition of anti-PreS1 and goat anti-human HBsAg blocking method, the coincidence rate of the two methods is 96%, but the latter is more sensitive. Anti-PreS1 titers up to 1: 1280 were measured using goat anti-human HBsAg blocking assay.