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目的:探讨干扰素诱导跨膜蛋白3基因(IFITM3)多态性与小儿流感易感性及严重性的关系。方法:选取2015年1—11月期间收治确诊的流感患儿60例(普通流感30例和重症流感30例)作为观察组,另选以门诊体检正常儿童60例作为对照组,提取两组患者DNA并扩增相应的目的片段后,采用特异性引物聚合酶链反应技术检测IFITM3 rs12252T/C的单核苷酸多态性,并进行统计学分析。结果:观察组患者中IFITM3基因rs12252 CT基因型明显多于对照组(P<0.05);C、T等位基因频率在观察组和对照组、普通流感、重症流感的分布经比较其差异均无统计学意义(P>0.05);在显性遗传模式中,观察组和对照组中(CC+CT)和TT基因型携带者的分布经比较其差异有统计学意义(P<0.05);在隐性遗传模式中,观察组和对照组中CC和(TT+CT)基因型携带者的分布经比较其差异无统计学意义(P>0.05);在共显性遗传模式分析中,观察组和对照组中CT和(TT+CC)基因型的比例经比较其差异有统计学意义(P<0.05)。结论:IFITM3基因rs12252 CT基因型可能是儿童流感的遗传易感因子,但C、T等位基因与儿童流感严重性无相关性。
Objective: To investigate the relationship between interferon-inducible transmembrane protein 3 (IFITM3) polymorphisms and susceptibility and severity of influenza in children. Methods: Sixty infants diagnosed with influenza (30 cases of common flu and 30 cases of severe flu) were selected as the observation group from January to November in 2015. Another 60 normal children in the outpatient examination were selected as the control group. Two groups of patients DNA and amplification of the corresponding target fragment, using a specific primer polymerase chain reaction detection IFITM3 rs12252T / C single nucleotide polymorphisms, and statistical analysis. Results: The rs12252 CT genotype of IFITM3 gene was significantly higher in the observation group than that in the control group (P <0.05). The frequency of C and T allele in observation group and control group was significantly lower than that in control group Statistical significance (P> 0.05). In the dominant genetic model, the distributions of CC + CT and TT genotypes in the observation group and the control group were statistically significant (P <0.05) In the recessive model, the distribution of CC and (TT + CT) carriers in the observation group and the control group showed no significant difference (P> 0.05). In the co-dominant genetic model analysis, the observation group There were significant differences in the ratio of CT and (TT + CC) genotypes between the control group and the control group (P <0.05). CONCLUSION: CT genotype of IFITM3 rs12252 may be a genetic predisposing factor for childhood influenza, but there is no correlation between C and T alleles and the severity of childhood flu.