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目的 :探讨睫状神经营养因子对应激引起海马 CA3区神经元损害的作用。方法 :采用 Bielschowsky- Gros-Lawrentjew染色法和常规透射电镜技术 ,观察急性和慢性足底电击大鼠的海马 CA3区神经元形态的变化 ,及双侧海马注射睫状神经营养因子对其影响。结果 :急性应激大鼠海马神经元形态无明显变化 :慢性应激大鼠海马神经元出现明显的损伤性形态变化 ;睫状神经营养因子对正常大鼠和急性应激大鼠的海马神经元形态无明显作用 ,但可显著减轻海马神经元损伤程度。结论 :睫状神经营养因子可能通过保护海马 CA3区神经元从而改善慢性应激大鼠的行为障碍
Objective: To investigate the effect of ciliary neurotrophic factor on neuron damage induced by stress in hippocampal CA3 region. Methods: Bielschowsky-Gros-Lawrentje staining and conventional transmission electron microscopy were used to observe the morphological changes of hippocampal CA3 neurons in acute and chronic foot-shock rats and the effect of bilateral ciliary neurotrophic factor injection on them. Results: There was no significant change in the morphology of hippocampal neurons in acute stress rats: The morphological changes of hippocampal neurons in chronic stress rats were obvious. The effects of ciliary neurotrophic factor on hippocampal neurons in normal rats and acute stress rats There is no obvious effect on the morphology, but it can significantly reduce the damage of hippocampal neurons. CONCLUSION: Ciliary neurotrophic factor may improve behavioral disorders in chronic stress rats by protecting neurons in hippocampal CA3 region