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作者用一种人肝瘤细胞(HuH-7)研究了反义(antisense)寡核苷酸对乙型肝炎病毒基因表达和复制的作用。第一组瘤细胞用HBV-DNA转染作为对照,第二组瘤细胞用一种反义寡核苷酸(ATC-40)与HBV-DNA共转染,第三组瘤细胞用同一段顺义(sense)寡核苷酸(GAT-40)与HBV-DNA
The authors used a human hepatoma cell line (HuH-7) to study the effect of antisense oligonucleotides on hepatitis B virus gene expression and replication. The first group of tumor cells was transfected with HBV-DNA as a control. The second group of tumor cells were co-transfected with HBV-DNA with an antisense oligonucleotide (ATC-40), and the third group of tumor cells were infected with the same section of Shunyi sense oligonucleotide (GAT-40) and HBV-DNA