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目的:探讨p53和Ki-67基因表达与膀胱癌病理分级和临床分期的关系。方法:回顾性分析2013年1月至2015年1月在本院与天津医科大学第二医院收治的445例膀胱癌患者的临床资料，应用免疫组织化学染色的方法，对这些膀胱癌患者的病理切片进行p53和Ki-67免疫组化染色，并将p53和Ki-67基因的免疫组化表达强度结果与患者的病理分级和临床分期进行分析。结果:在445例膀胱癌患者中，435例患者测得p53免疫组化指标，440例患者测得Ki-67免疫组化指标，Spearman秩相关分析表明，p53和Ki-67阳性程度越大，患者膀胱癌的病理分级、临床分期越高(n P<0.05)。其中p53、Ki-67与病理分级的相关系数分别为0.298、0.181，与临床分期的相关系数分别为0.143、0.103，均呈正相关。n 结论:p53和Ki-67基因的表达强度与膀胱癌病理分级与临床分期密切相关，即阳性程度越大，病理分级、临床分期越高。“,”Objective:To evaluate the relationship of the expression of p53, Ki-67 and bladder cancer stage and grade.Methods:Retrospective analysis of the clinical data of 445 patients with bladder cancer in our hospital and the second hospital of Tianjin medical university was performed.The expression intensity of p53 and Ki-67 in specimens from 445 patients of bladder cancer was studied by means of immunohistochemistry.The results were analyzed in relation to the tumor stage and grade.Results:In 445 cases of bladder cancer patients, p53 were examined in 435 patients, Ki-67 were examined in 440 patients.On Spearman rank correlation analysis, the higher expressions of p53 and Ki-67, the higher pathological grading and clinical staging of bladder cancer in patients(n P<0.05), the correlation coefficients of them in relation to pathological grading were 0.298, 0.181, and the correlation coefficients of them in relation to clinical staging were 0.143, 0.103, all positive correlations.n Conclusions:The expression intensities of p53 and Ki-67 are closely related to bladder cancer pathological grading and clinical staging, the higher expressions of p53 and Ki-67, the higher pathological grading and clinical staging of bladder cancer in patients.