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研究了不同粒径的大气细颗粒物对人肺癌上皮细胞A549的生物学效应,探讨了大气中细颗粒物粒径与细胞毒性的关系。采集杭州市大气中不同粒径的细颗粒物,制备成不同粒径的颗粒物总悬浮液,将A549细胞暴露于该制备液24 h后,测定细胞活力(MTT法)和培养液上清液中的LDH含量;并选择最佳浓度为后续暴露剂量,测定ROS生成量,评价不同粒径大气细颗粒物对细胞损伤效应。此外,还采用RTPCR测定凋亡基因P50、BAX、BCL-2表达情况。不同粒径的细颗粒物均显著抑制A549细胞活力,LDH显著增多,并存在剂量-效应关系;最佳染毒质量浓度为10μg/m L;ROS的生成量和各个凋亡基因的mRNA表达量均高于对照组。大气中不同粒径的细颗粒物均能对A549细胞产生毒性,并且随粒径增加,颗粒物对细胞的毒性呈降低趋势。
The biological effect of airborne fine particles with different particle size on human lung cancer cell line A549 was studied, and the relationship between the particle size and cytotoxicity was discussed. After the A549 cells were exposed to the preparation solution for 24 h, the cell viability (MTT assay) and the supernatant of the culture supernatant were measured LDH content was determined. The optimal dose of ROS was selected as follow-up dose, and the ROS production was measured to evaluate the effect of airborne particulate matter of different particle size on cell injury. In addition, the expression of apoptosis gene P50, BAX, BCL-2 was also determined by RTPCR. The fine particles with different particle size significantly inhibited the viability of A549 cells, with a significant increase in LDH, and there was a dose-effect relationship. The best concentration of 10μg / ml Lactobacillus was used to treat A549 cells. The production of ROS and the mRNA expression of all apoptosis genes Higher than the control group. A549 cells could be toxic to A549 cells with different particle size in the atmosphere. With the increase of particle size, the toxicity of the particles to the cells decreased.