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目的探讨防治节育器(IUD)出血的新方法,研究血管内皮生长因子(VEGF)蛋白对置节育器后出血副反应的疗效及对内膜微血管生成的影响。方法收集60例研究对象,将VEGF蛋白于月经期经导管置入宫腔底部进行灌注,每月1次,共3次。收集置器者的卫生巾及子宫内膜,采用月经图示法评估月经血量,采用月经出血模式统计方法分析出血模式,采用免疫组化法检测内膜VEGF的表达及微血管密度(MVD)。结果 VEGF组滴血天数为(11.8±4.9)d,与抗前列腺素组(23.4±11.7)d和对照组(25.2±10.6)d比较,明显缩短,差异有统计学意义(P<0.05)。出血天数、月经周期长度、月经图示评分差异无统计学意义(P>0.05)。三组MVD置器后与置器前自身对照均有升高,差异有统计学意义(P<0.05),VEGF组置器后微血管密度与抗前列腺素组、对照组比较显著升高,差异有统计学意义(P<0.05)。三组VEGF表达置器后与置器前自身对照均有升高,差异有统计学意义(P<0.05)。置器后三组间两两比较,差异无统计学意义(P>0.05)。结论 VEGF蛋白宫腔灌注可能缩短置器后滴血时间,与VEGF蛋白促进内膜微血管生成有关。
Objective To explore a new method of prevention and treatment of IUD bleeding and to investigate the curative effect of vascular endothelial growth factor (VEGF) protein on hematologic malignancies after hemorrhage and its effect on endometrial microangiogenesis. Methods Sixty subjects were collected. The VEGF protein was infused into the uterine cavity at the bottom of the uterine cavity during menstruation, once a month for 3 times. The sanitary napkins and endometrium were collected. The menstrual flow was evaluated by the menstruation diagram. The bleeding patterns were analyzed by the menstrual bleeding pattern. The expression of VEGF and the microvessel density (MVD) were detected by immunohistochemistry. Results The number of days of blood drip in VEGF group was (11.8 ± 4.9) d, which was significantly shorter than that in anti-prostaglandin group (23.4 ± 11.7) d and control group (25.2 ± 10.6) d, the difference was statistically significant (P <0.05). Bleeding days, menstrual cycle length, menstrual graphics showed no significant difference (P> 0.05). After three sets of MVD device and the device before the self-control were increased, the difference was statistically significant (P <0.05), microvessel density after VEGF organizer anti-prostaglandin group compared with the control group was significantly higher, the difference was Statistical significance (P <0.05). After three sets of VEGF expression setter and pre-device self-control were increased, the difference was statistically significant (P <0.05). There was no significant difference between the two groups (P> 0.05). Conclusion Intrauterine perfusion of VEGF protein may shorten the time of blood transfusion after implantation, which is related to the VEGF protein promoting the intimal microangiogenesis.