论文部分内容阅读
目的 研究花椒油素对CaCl2和钙离子载体A23187调节血小板及其中Ca2+浓度的作用。方法 比浊法测定血小板聚集，用Fura-2/AM荧光法测定细胞内游离钙的水平。结果 花椒油素显著地抑制CaCl2和A23187诱导的血小板聚集，前者的抑制率为9.0%(67.1%，后者的抑制率为18.1%(72.5%。明显降低A23187诱导的血小板胞质游离Ca2+浓度，降低率为12.8%(63.4%。结论 花椒油素抑制聚集作用与降低细胞内游离Ca2+水平之间呈线性正相关（P<0.01）。
Objective To study the effect of ginkgo on the regulation of platelet and Ca2+ concentration in CaCl2 and calcium ionophore A23187. Methods The platelet aggregation was measured by turbidimetry, and the intracellular free calcium was measured by Fura-2/AM fluorescence method. Results The results showed that the inhibitory rate of platelet aggregation was significantly inhibited by CaCl2 and A23187. The inhibition rate of the former was 9.0% (67.1%), and the latter was 18.1% (72.5%). The platelet cytoplasmic free Ca2+ concentration induced by A23187 was significantly decreased. The rate of reduction was 12.8% (63.4%).Conclusion There was a linear positive correlation between the inhibitory aggregation of gambopol and the decrease of intracellular free Ca2+ levels (P<0.01).