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目的:研究补骨脂素对人成骨肉瘤MG-63细胞增殖的影响,以探讨其对成骨肉瘤细胞的生物学效应。方法:以640μmol/L补骨脂素处理人成骨肉瘤MG-63细胞,通过生长曲线测定,流式细胞仪分析和光镜观察方法,研究补骨脂素对MG-63细胞的生长曲线和细胞周期的影响,并对其作用机理进行初步分析。结果:补骨脂素处理72h后,MG-63细胞生长抑制率达到77.74%,G0/G1期比例达到55.8%,细胞生长活性减退,体积变小、变圆,伪足消失,悬浮、空泡化现象明显。结论:640μmol/L补骨脂素可以有效抑制细胞的增殖活动,改变细胞形态特征,对细胞具有凋亡作用。
Objective: To study the effect of psoralen on the proliferation of human osteosarcoma MG-63 cells and to explore its biological effects on osteosarcoma cells. METHODS: Human osteosarcoma MG-63 cells were treated with 640 μmol/L psoralen. Growth curve, flow cytometric analysis and light microscopy were performed to study the growth curve and cells of psoralen on MG-63 cells. The impact of the cycle, and a preliminary analysis of its mechanism of action. RESULTS: After treatment with psoralen for 72 h, the growth inhibition rate of MG-63 cells reached 77.74%, the ratio of G0/G1 phase reached 55.8%, the cell growth activity decreased, the volume became smaller and rounder, the pseudopodia disappeared, and the suspension and vacuolization were observed. The phenomenon is obvious. Conclusion: 640 μmol/L psoralen can effectively inhibit the proliferation of cells, change the morphology of cells and have apoptotic effect on cells.