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目的探讨血小板源性生长因子受体(platelet-derived growth factor receptor alpha,PDGFRA)在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中的表达情况及其临床应用价值。方法用免疫组化SP法检测78例GIST中PDGFRA及CD117的表达情况,同时观察PDGFRA在其它GI MT中的表达情况。结果PDGFRA及CD117在GIST中阳性表达率分别为51%(40/78)及92%(72/78);PDGFRA在CD117阴性GIST中表达率为83%(5/6),在CD117阳性GIST中的表达率为49%(35/72)。PDGFRA及CD117的表达分别在组织学分型之间比较差异均有显著性(P<0.05),而在不同原发部位及生物学行为分组之间比较差异均无显著性(P>0.05)。PDGFRA在7例消化道神经鞘瘤中3例呈阳性表达,在4例胃肠道平滑肌瘤、9例平滑肌肉瘤、2例横纹肌肉瘤、2例纤维瘤病中均为阴性表达。结论PDGFRA可作为GIST诊断的有用标记物,尤其是在CD117阴性GIST的诊断及鉴别诊断中具有重要的临床意义。
Objective To investigate the expression of platelet-derived growth factor receptor alpha (PDGFRA) in gastrointestinal stromal tumors (GIST) and its clinical value. Methods The expressions of PDGFRA and CD117 in 78 cases of GIST were detected by immunohistochemical SP method. Meanwhile, the expression of PDGFRA in other GI MTs was also observed. Results The positive rates of PDGFRA and CD117 in GIST were 51% (40/78) and 92% (72/78), respectively. The positive rate of PDGFRA in CD117 negative GIST was 83% (5/6) The expression rate was 49% (35/72). The expressions of PDGFRA and CD117 were significantly different between the histological types (P <0.05), but not between the different primary sites and the biological behavior groups (P> 0.05). PDGFRA was positive in 7 cases of gastrointestinal schwannoma, and negative in 4 cases of gastrointestinal leiomyoma, 9 cases of leiomyosarcoma, 2 cases of rhabdomyosarcoma and 2 cases of fibromatosis. Conclusion PDGFRA can be a useful marker of GIST diagnosis, especially in the diagnosis and differential diagnosis of CD117 negative GIST.