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Objective To investigate the induction cytotoxic T cells (CTLs) with antitumor activity and therapeutic efficacy after dendritic cells(DCs) acquired antigen from apoptotic cholangiocarcinoma cells caused by y-irradiation.Methods DCs from peripheral blood mononuclear cells (PBMC) that maintain the antigen capturing and processing capacity charateristicof immature cells have been established in vitro, using granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Then, in cholangiocarcinoma cells apoptosis was induced by J-irradiation. The experimental groups were as follows: (1) coculture ofDCs and apoptotic cancer cells and T cells; (2) coculture of DCs and necrotic cancer cells and T cells; (3) coculture of DCs, culturedcancer cell and T cells. They are cocultured for 7 days. DCs and T cells were riched, isolated and their antitumor response was tested.Results The cells had typical dendritic morphology, expressed high levels of GDI a and B7, acquired antigen from apoptotic ce
Objective To investigate the induction of cytotoxic T cells (CTLs) with antitumor activity and therapeutic efficacy after dendritic cells (DCs) acquired antigen from apoptotic cholangiocarcinoma cells caused by y-irradiation. Methods DCs from peripheral blood mononuclear cells and processing capacity charateristicof immature cells have been established in vitro, using granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Then, in cholangiocarcinoma cells apoptosis was induced by J- irradiation. The experimental groups were as follows: (1) coculture of DCs and apoptotic cancer cells and T cells; (2) coculture of DCs and necrotic cancer cells and T cells; (3) coculture of DCs, cultured cancer cells and T cells. They are cocultured for 7 days. DCs and T cells were riched, isolated and their antitumor response was tested. Results The cells had typical dendritic morphology, expressed high levels of GDI a and B7, acquired antigen from apoptotic ce