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单个病毒的三维操作对自下而上开展单分子尺度的细胞免疫学研究具有重要的意义,然而受操作工具及操作方法的限制,该问题一直未能得到很好的解决.以基于原子力显微镜的纳米操作机器人为基础,开展了针对单个病毒分子的三维操作研究,具体包括:高均匀分散性病毒样品制备方法,基于局部扫描的病毒三维操作结果实时检测策略,以及微观尺度下病毒三维空间的定点放置实现.实验结果表明,本文提出的操作方法与技巧,不仅能够实现单个病毒分子在三维空间内的可控定点拾取与释放,还可与二维操作方法相结合,构建出全病毒分子的三维纳米结构,实验结果验证了所提方法的正确性和有效性,从而为自下而上的细胞免疫学开展提供了可行的技术途径.
The three-dimensional operation of a single virus is of great significance for the study of single-cell-scale cellular immunology from bottom to top, however, this problem has not been well solved due to the limitation of operating tools and operation methods.Based on atomic force microscopy Based on the nanomanipulator, three-dimensional operation research on single virus molecule has been carried out, including the preparation of highly uniform and dispersible virus samples, the real-time detection strategy of virus three-dimensional operation results based on local scanning and the fixed point of virus three-dimensional space on the microscopic scale The experimental results show that the method and technique proposed in this paper can not only realize the controllable point-picking and releasing of a single virus molecule in three-dimensional space, but also combine the two-dimensional operation method to construct the three-dimensional The experimental results verify the correctness and validity of the proposed method, which provides a feasible technical approach for bottom-up cellular immunology.