本文报道融合后一步选择和克隆杂交瘤的简单方法,此法比常规的有限稀释法更易获得大量细胞和收获许多独立的杂交瘤克隆。这样既不需要再克隆化,又省去了大量组织培养。所以本技术特别适用于分离大量的杂交瘤,筛选得到不同的单克隆抗体。 This article reports a simple method of selecting and cloning hybridomas in one step after fusion, which is more accessible to a large number of cells and harvests many independent hybridoma clones than the conventional limited dilution method. This neither need to be cloned, but also save a lot of tissue culture. Therefore, the technique is particularly suitable for isolating a large number of hybridomas and selecting different monoclonal antibodies.