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本文利用ＲＡＰＤ技术对我国２８个玉米骨干自交系进行了分析，从３００个引物中筛选出了４０个多态性好的引物，结果发现：仅使用ＯＰＩ１０一个引物扩增的ＤＮＡ指纹图谱就能区别开１５个自交系，增加引物ＯＰＢ７可以区分开２６个自交系，再增加ＯＰＣ１９、ＯＰＧ６、ＯＰＡ７、ＯＰＩ１１、ＯＰＧ１４中的任何一个引物就可以将２８个自交系完全区分开。研究还表明亲缘关系很近、利用同工酶方法无法区分的姊妹系材料如：４７８、４８８和３１８９，以及双１０５和双７４１都能够被明显的区分开，证明了利用ＲＡＰＤ技术进行玉米自交系鉴定完全可行而且是非常有效的。
In this paper, 28 maize skeleton inbred lines in China were analyzed by RAPD technique, and 40 primers with good polymorphism were screened out from 300 primers. The results showed that DNA fingerprinting amplified with only one primer of OPI10 could A total of 15 inbred lines were distinguished from each other, and 28 inbred lines could be distinguished by the addition of primer OPB7. Any one of primers OPC19, OPG6, OPA7, OPI11 and OPG14 could be used to distinguish 28 inbred lines completely. The study also showed that close relatives, sister lines such as 478, 488 and 3189 that could not be differentiated using the isoenzyme method, as well as double 105 and double 741, were clearly distinguishable, demonstrating the use of RAPD for selfing corn The identification is entirely feasible and very effective.