将未处理的（对照）和重铬酸钾处理的Ｖ７９细胞贮存于４℃、－２０℃、－８０℃和－１９６℃，于保存后的０，４，２４，４８，１６８ｈ取出进行单细胞凝胶电泳（ＳＣＧＥ）试验。结果显示各温度保存２４ｈ的细胞ＤＮＡ迁移长度与新鲜细胞无差异；更长时间（４８和１６８ｈ）的保存细胞ＤＮＡ迁移长度明显增加；同时各温度保存的细胞存活率均随贮存时间的延长而下降。因此，２４ｈ内细胞保存以４℃为好，而２４ｈ以上的保存则以－８０℃为佳 Untreated (control) and potassium dichromate-treated V79 cells were stored at 4 ° C, -20 ° C, -80 ° C and -196 ° C, taken out at 0,4,24,48,168 h after storage for single cell Gel electrophoresis (SCGE) test. The results showed that there was no difference between the cell migration length and the fresh cells at each temperature for 24 hours. The DNA migration length of the preserved cells increased obviously at longer time (48 and 168 hours), while the cell survival rate at each temperature decreased with the prolongation of storage time . Therefore, 24h preservation of cells at 4 ℃ is better, while the preservation of more than 24h at -80 ℃ is better