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目的 探讨放射性核素对培养平滑肌细胞增殖及凋亡的作用及其机制 ,观察放射性核素支架预防再狭窄的可行性及有效性。方法 将培养平滑肌细胞分为188Re照射组与非照射组 ,应用细胞及分子生物学技术 ,研究了放射性核素188Re对离体平滑肌细胞增殖、凋亡、次黄嘌呤鸟嘌呤磷酸核糖转移酶 (HPRT)基因突变的作用 ;将兔损伤髂动脉内随机放置放射性支架与非放射性支架 ,观察了放射性核素支架对动物血管再狭窄模型的预防作用。结果 与非照射组比较 ,188Reβ辐射 (放射比活度为 0 74、1 4 8、2 2 2、2 96、3 70GBq/L)能够明显抑制平滑肌细胞的增殖 ,抑制细胞增殖率 (1 4 8～3 70GBg/L) [(10 0 0 %vs 34 1% ,t =2 5 0 0 ,P <0 0 5 ) ,(10 0 0 %vs 2 7 7% ,t=2 74 4 ,P <0 0 5 ) ,(10 0 0 %vs 2 1 8% ,t=2 972 ,P <0 0 5 ) ,(10 0 0 %vs 18 1% ,t=3 12 1,P <0 0 5 ) ],阻滞细胞进入DNA合成期 { [(43 5 6± 2 32 ) %vs (2 3 80± 2 5 7) % ,t =11 4 16 ,P <0 0 0 1],[(43 5 6± 2 32 ) %vs(11 82± 2 5 1) % ,t =18 5 6 6 ,P <0 0 0 1],[(43 5 6± 2 32 ) %vs (6 0 4± 1 2 0 ) % ,t =2 8 734,P <0 0 0 1],[(43 5 6± 2 32 ) %vs (3 30± 0 5 2 ) % ,t=33 892 ,P <0 0 0 1],[(43 5 6± 2
Objective To investigate the effect and mechanism of radionuclide on the proliferation and apoptosis of cultured smooth muscle cells and to observe the feasibility and effectiveness of radionuclide scaffolds in preventing restenosis. Methods The cultured smooth muscle cells were divided into 188Re irradiation group and non-irradiation group. The effects of 188Re on proliferation, apoptosis, hypoxanthine guanine phosphoribosyltransferase (HPRT) ) Gene mutations in rabbit iliac artery injury random placement of radioactive scaffolds and non-radioactive scaffolds, radionuclide scaffolds on animal vascular restenosis model was observed preventive effect. Results Compared with non-irradiated group, 188Reβ radiation (specific radioactivity of 0 74,1 4 8,2 2 2,2 96,3 70GBq / L) could significantly inhibit the proliferation of smooth muscle cells and inhibit the proliferation rate of cells ~ 70GBg / L) (100% vs 34%, t = 2500, P <0 05), (100% vs 277%, t = 2 744, P < (10 0 0 vs 18 1%, t = 3 12 1, P 0 05), (100% vs 2 1 8%, t = 2 972, P 0 05) ], Blocking the cell into the DNA synthesis period {[(435 ± 2 32)% vs (23 80 ± 257)%, t = 11 4 16, P <0 0 01], [(43 5 6 ± 2 32% vs 11 82 ± 2 5 1%, t = 18 566, P 0 01 0, and (43 56 ± 2 32)% vs (60 4 ± 1 2 T = 2 8 734, P <0 0 0 1], [(43 56 ± 2 32)% vs (30 30 ± 0 52)%, t = 33 892, P 0 01 0 ], [(43 5 6 ± 2