论文部分内容阅读
］目的：研究胆管Ｂｏｙｄｅｎ括约肌在胆汁排出中的作用、机理及调控作用。方法：１６只犬，随机分为对照组，实验组（胆囊切除组）。采用电影摄影，灌注法测压，对内源性缩胆素的反应，免疫组化定量分析及超微结构观察等方法进行研究。结果：胆管Ｂｏｙｄｅｎ括约肌为低压管腔，压力为（１．３３ ± ０．２７）ｋＰａ，明显低于Ｏｄｄｉ括约肌高压区基础压（２．２５±０．０６）ｋＰａ（Ｐ＜０．０１）。Ｂｏｙｄｅｎ括约肌具有自主收缩、舒张功能，但似不受内源性缩胆素的调控。胆囊切除后Ｂｏｙｄｅｎ括约肌ａ－Ａｃｔｉｎ和Ｍｙｏｓｉｎ含量与对照组间无明显差异（Ｐ＞０．０５），超微结构的改变主要为细胞骨架的三维空间结构排列紊乱，同时伴有细胞内线粒体肿胀和分布异常。结论：在禁食期间，胆管 Ｂｏｙｄｅｎ括约肌是胆汁排泄的主要动力源。 Ｂｏｙｄｅｎ括约肌运动功能以植物神经调节为主，完整存在的胆囊是保证 Ｂｏｙｄｅｎ括约肌和 Ｏｄｄｉ括约肌之间功能统一的基础。
Objective: To study the role, mechanism and regulation of biliary duct sphincter in bile excretion. Methods: 16 dogs were randomly divided into control group and experimental group (cholecystectomy group). Using cinematography, perfusion manometry, response to endogenous cholinergic, immunohistochemical quantitative analysis and ultrastructure observation methods. Results: The pressure of the Boyden sphincter in the bile duct was (1.33 ± 0.27) kPa, which was significantly lower than that of the Oddi sphincter (2.25 ± 0.06) kPa (P <0.01). Boyden sphincter with independent contraction, diastolic function, but may not be regulated by endogenous cholinergic. The contents of a-actin and Myosin in Boyden sphincter after cholecystectomy were not significantly different from those in control group (P> 0.05). The ultrastructural changes were mainly due to disordered three-dimensional spatial structure of cytoskeleton and mitochondrial swelling Abnormal distribution. Conclusion: During fasting, the biliary duct sphincter is the main source of biliary excretion. Boyden sphincter motor function mainly in autonomic regulation, the integrity of the gallbladder is to ensure that the sphincter of Boyden and Oddi sphincter between the basis of unity.