论文部分内容阅读
目的建立毛细管气相色谱法测定薄荷素油中(-)-薄荷酮和薄荷脑含量。方法采用毛细管气相色谱法,色谱柱为HP-INNOWAX(30 m×0.32 mm,0.25μm)弹性石英毛细管色谱柱,柱温120℃,检测器温度为250℃,进样口温度为200℃,载气为N2,流速为1 mL.min-1。结果(-)-薄荷酮和薄荷脑的回归方程分别为:Y=84.20X+210.49(r=0.999 2),Y’=84.68X’+295.97(r’=0.998 9);线性范围分别为0.6～3.6和0.8～4.8 g.L-1,平均回收率分别为101.5%,101.1%。结论方法简便、快速,结果准确、可靠,适用于薄荷素油的质量控制。
Objective To establish a capillary gas chromatographic method for the determination of (-)-menthone and menthol in peppermint oil. Methods Capillary gas chromatography was used. The column was HP-INNOWAX (30 m×0.32 mm, 0.25 μm) elastic quartz capillary column. The column temperature was 120°C. The detector temperature was 250°C. The inlet temperature was 200°C. The gas is N2 and the flow rate is 1 mL.min-1. Results The regression equations of (-)-menthone and menthol were: Y=84.20X+210.49 (r=0.999 2), Y’=84.68X’+295.97 (r’=0.998 9); the linear range was 0.6. At -3.6 and 0.8-4.8 gL-1, the average recoveries were 101.5% and 101.1%, respectively. Conclusion The method is simple, rapid, accurate, reliable, and suitable for the quality control of peppermint oil.