论文部分内容阅读
建立了亚甲基蓝(MB)修饰多壁碳纳米管玻碳电极(MWNT/GCE)测定药片中己烯雌酚(DES)的方法,并采用非现场型傅里叶红外光谱技术(Off-site-FTIR)对亚甲基蓝修饰电极进行了表征检测。结果表明,聚亚甲基蓝在电极上的形成是由于双键断裂,使得在1603.3cm~(-1)处的C=N键伸缩振动吸收带消失;在0.2mol/L的磷酸缓冲液(pH=7.5)中,采用修饰电极己烯雌酚的伏安曲线出现特征氧化峰,峰电位为0.209V,峰电流显著提高,表现出明显的电催化作用;利用方波伏安法(Square WareVoltammetry,SWV)测得峰电流与己烯雌酚质量浓度在1.0×10~(-5)—1.0×10~(-9)g/L范围内呈线性关系,线性相关系数0.9949,检出限5.0×10~(-10)g/L。用此法对己烯雌酚药片的含量进行了测定,测定结果与标示相符。
A method for the determination of diethylstilbestrol (DES) in tablets by methylene blue (MB) modified MWNT / GCE was established. The off-site-FTIR spectra of methylene blue Modified electrodes were characterized for detection. The results showed that the formation of methylene blue on the electrode was due to the cleavage of the double bond, resulting in the disappearance of the C = N stretching vibration absorption band at 1603.3 cm -1. In 0.2 mol / L phosphate buffer (pH = 7.5 ), The characteristic oxidation peak of voltammogram of diethylstilbestrol at the modified electrode appeared. The peak potential was 0.209V, the peak current was significantly increased, showing obvious electrocatalytic activity. The peak value was measured by square wave voltammetry (SWV) The linear relationship between the concentration of diethylstilbestrol and the concentration of diethylstilbestrol in the range of 1.0 × 10 -5 -1.0 × 10 -9 g / L, the linear correlation coefficient of 0.9949, the detection limit of 5.0 × 10 -10 g / L. With this method of diethylstilbestrol tablets were determined, the determination of the results and the mark.