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目的:分析微小核糖核酸(mi RNA)let-7c通过靶定细胞周期蛋白依赖激酶6(CDK6)抑制肝癌细胞增殖的临床意义，并探讨其对肝癌细胞增殖的影响以及靶基因。方法:选取人低转移肝癌细胞-97L(MHCC-97L)、人高转移肝癌细胞(HCCLM3)、人肝癌组织(HepG2)、人肝癌细胞-7721(SMMC-7721)、人肝细胞LO2、小鼠抗人细胞周期蛋白依赖激酶抗体等进行细胞培养，利用Western blot检测转染后小鼠CDK6表达情况，转染48 h后应用细胞裂解液对三组蛋白进行提取，将材料分为观察组、对照组、空白组，观察组转染let-7c，对照组转染阴性对照miRNA，空白组未进行转染处理；应用二喹啉甲酸法对各孔细胞蛋白浓度进行测定。结果:let-7c对肝癌细胞HCCLM3增殖结果显示，于450 nm处观察组、对照组转染后24 h吸光度值差异无统计学意义(n P>0.05)；转染48 h后观察组、对照组HCCLM3细胞中CDK6蛋白含量发生较大变化，观察组的CDK6蛋白表达量为(0.58±0.05)，均低于对照组的(0.81±0.08)与空白组(0.85±0.09)(n t＝10.107、13.876，均n P0.05). After 48 hours of transfection, the content of CDK6 protein in the observation group and the control group changed greatly, and the expression of CDK6 protein in the observation group(0.58±0.05) was lower than that in the control group(0.81±0.08) and blank group (0.85±0.09)(n t=10.107, 13.876, all n P<0.05). At 72 hours after transfection, the percentage of cells in G1 phase in the observation group [(56.21±3.25)%] was significantly higher than that in the control group [(45.21±1.56)%] and blank group [(46.24±1.98)%], the differences were statistically significant (n t=16.146, 13.862, all n P=0.000).n Conclusion:The expression of let-7c in hepatocellular carcinoma cells is low.Upregulation of let-7c can effectively regulate CDK6, thus inhibiting the growth of cancer cells, and has a significant inhibitory effect on the proliferation of hepatocellular carcinoma cells.