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目的探讨斑马鱼急性毒性检测平台在食品安全性检测中的应用性。方法通过完善和优化斑马鱼驯养条件、斑马鱼急性试验用鱼要求、斑马鱼急性毒性试验条件建立斑马鱼急性毒性检测平台,检测和分析不同浓度的硫氰酸钠、蛹虫草子实体水提物和醇提物对斑马鱼的毒性作用,分别观察和记录斑马鱼在受试物的试验溶液中暴露48h的毒性反应和死亡情况。结果斑马鱼在剂量为1000 mg/L和800 mg/L的硫氰酸钠试验溶液饲养1 h后开始出现毒性反应,在剂量为600 mg/L和400 mg/L的硫氰酸钠试验溶液饲养4h后开始出现毒性反应,硫氰酸钠对斑马鱼的48h LC5 0为572.4 mg/L。斑马鱼在浓度为100~1 000 mg/L的蛹虫草子实体水提物试验溶液中饲养48h,未发现斑马鱼有任何毒性反应,蛹虫草子实体水提物对斑马鱼的48h LC5 0大于1 000 mg/L;斑马鱼在浓度为100~1 000 mg/L的蛹虫草子实体醇提物试验溶液中饲养48h也未发现斑马鱼有任何毒性反应,蛹虫草子实体醇提物对斑马鱼的48h LC5 0大于1000mg/L。结论斑马鱼急性毒性检测平台能够用于食品和食品污染物急性毒性的快速检测,该检测平台将在食品的安全性检测中有较好的应用前景。
Objective To investigate the application of zebrafish acute toxicity testing platform in food safety testing. Methods The zebrafish acute toxicity test platform was established by perfecting and optimizing zooplankton conditions of zebrafish, zebrafish test fish, and zebrafish acute toxicity test. The aqueous extracts of sodium dicyanzate and Cordyceps militaris were tested and analyzed. And alcohol extract on zebrafish toxicity were observed and recorded in the test substance zebrafish test solution exposure 48h toxicity and death. Results The zebrafish began to show toxicity after 1 h of incubation with the test solutions of 1000 mg / L and 800 mg / L sodium thiocyanate. At 600 mg / L and 400 mg / L of sodium thiocyanate test solution Toxicity began to occur 4h after feeding, and the 48h LC50 of sodium thiocyanate on zebrafish was 572.4 mg / L. The zebrafish were incubated in the test solution of the aqueous extract of Cordyceps militaris fruiting body at a concentration of 100-1000 mg / L for 48 h. No zebrafish toxic reaction was found. The 48 h LC50 of zebrafish was higher than 1 000 mg / L; zebrafish in the concentration of 100 ~ 1 000 mg / L Cordyceps militaris alcohol extract test solution incubated 48h also did not find any toxic reaction of zebrafish, Cordyceps militaris alcohol extract of zebra Fish 48h LC50 is greater than 1000mg / L. Conclusion The zebrafish acute toxicity test platform can be used for the rapid detection of acute toxicity of food and food contaminants. The detection platform will have a good application prospect in food safety testing.