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目的:探讨胸腺基质淋巴细胞生成素(TSLP)在变应性鼻炎模型小鼠鼻黏膜中的表达。方法:将20只6~8周BALB/c小鼠随机分为变应性鼻炎组和自然对照组,每组10只。以鸡卵清蛋白致敏建立变应性鼻炎模型。组织标本石蜡包埋,采用免疫组织化学方法和荧光PCR方法检测TSLP在小鼠鼻黏膜中的表达。结果:自然对照组鼻黏膜未见明显TSLP表达,变应性鼻炎组鼻黏膜可见明显TSLP表达。结论:TSLP在变应性鼻炎的鼻黏膜中表达。
Objective: To investigate the expression of thymic stromal lymphopoietin (TSLP) in the nasal mucosa of allergic rhinitis model mice. Methods: Twenty BALB / c mice aged 6 to 8 weeks were randomly divided into allergic rhinitis group and natural control group, with 10 rats in each group. Establishment of Allergic Rhinitis Model by Chicken Ovalbumin Sensitization. Tissue samples were embedded in paraffin, and the expression of TSLP in nasal mucosa of mice was detected by immunohistochemistry and fluorescent PCR. Results: There was no obvious TSLP expression in the nasal mucosa of the natural control group. The expression of TSLP in the nasal mucosa of allergic rhinitis group was obvious. Conclusion: TSLP is expressed in the nasal mucosa of allergic rhinitis.