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以蛹虫草(Cordyceps militaris(L.)Link)NS-810为菌种,通过对接种量的考察,探索不同孢子浓度对蛹虫草液体一级种制作效果的影响;通过单次单因子试验和正交实验,优化深层发酵产虫草素的最佳培养基,筛选制备蛹虫草液体一级种和液体发酵生产虫草素的最佳工艺。结果表明:孢子浓度3.0×10~8 cfu·mL~(-1)时制作的母种最适合作为蛹虫草菌种扩大培养中的一级种子液;深层发酵的最佳培养基配方为葡萄糖25g·L~(-1)、土豆100g·L~(-1)、鱼蛋白胨18g·L~(-1)、(NH_4)_2SO_40.8g·L~(-1)、KH_2PO_41.0g·L~(-1)、MgSO_4·7H_2O 0.5g·L~(-1)、蚕蛹粉5.0g·L~(-1)、维生素B118mg·L~(-1)、水1L。优化后虫草素的总产量为1 144.31mg·L~(-1),较基础培养基提高了1.46倍。分别以价格低廉葡萄糖和鱼蛋白胨作为发酵培养基的碳源和有机氮源,利于蛹虫草产业化发酵生产虫草素。
The Cordyceps militaris (L.) Link NS-810 strain was used as a strain to study the effects of different spore concentrations on the production of the first-class Cordyceps militaris liquid by inoculation inoculation. The single-factor test and positive The optimum medium for the production of cordycepin by submerged fermentation was optimized and the optimum conditions were established. The results showed that the optimum culture medium for the deep fermentation was 25g of glucose · L -1, 100 g · L -1, 18 g · L -1, (NH 4) 2 SO 40.8 g · L -1, KH 2 PO 41.0 g · L -1 -1), MgSO 4 · 7H 2 O 0.5g · L -1, pupa powder 5.0g · L -1, Vitamin B 118mg · L -1 and water 1L. The optimized yield of cordycepin was 1 144.31 mg · L -1, which was 1.46 times higher than that of basal medium. Respectively low-cost glucose and fish peptone as the fermentation medium carbon source and organic nitrogen source, which will help Cordyceps militaris industrial fermentation of cordycepin production.