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目的研究激酶功能区受体(KDR)基因表达沉默后对前列腺癌PC-3细胞裸鼠体内成瘤能力的影响。方法将15只5周龄BALB/c雄性裸鼠随机分为干扰组、阴性质粒组和未转染组,每组5只。分别接种构建的pSilencer 3.1-KDR siRNA表达质粒转染PC-3细胞、阴性对照质粒pSilencer3.1-NC转染PC-3细胞以及未转染的PC-3细胞于裸鼠皮下;观察各组PC-3细胞在裸鼠体内成瘤率、瘤体生长速度以及平均瘤质量等方面的变化,RT-PCR和Western blot技术检测瘤体KDR基因和蛋白表达。结果pSilencer3.1KDR质粒转染组的裸鼠瘤体生长速度明显慢于未转染组和pSilencer3.1-NC质粒转染组;与未转染组和PSilencer3.1-NC组相比,pSilencer3.1-KDR组肿瘤的生长受到明显抑制,平均体积较小(0.28 cm3vs 0.721 cm3,0.715 cm3,P<0.01),平均瘤质量较轻(0.14 g vs 0.648 g,0.635 g,P<0.01);裸鼠肿瘤组织中KDR mRNA和蛋白的表达明显降低。结论 RNAi介导的KDR基因沉默可显著影响PC-3细胞裸鼠体内的瘤体生长速度,KDR有可能成为肿瘤治疗的新靶点。
Objective To investigate the effect of KDR gene silencing on the tumorigenicity of prostate cancer PC-3 cells in nude mice. Methods Fifteen 5-week-old male BALB / c nude mice were randomly divided into interference group, negative plasmid group and untransfected group. The pSilencer 3.1-KDR siRNA expression plasmids were inoculated into PC-3 cells respectively and the negative control plasmid pSilencer3.1-NC was transfected into PC-3 cells and untransfected PC-3 cells in nude mice. PC 3 cells in nude mice in vivo tumor growth rate, tumor growth rate and average tumor mass changes, RT-PCR and Western blot detection of tumor KDR gene and protein expression. Results The growth rate of pSilencer3.1KDR plasmid-transfected group was significantly slower than that of untransfected group and pSilencer3.1-NC plasmid transfected group. Compared with untransfected group and PSilencer3.1-NC group, pSilencer3. The average tumor volume was significantly lower in the 1-KDR group (mean 0.28 cm3 vs 0.721 cm3, 0.715 cm3, P <0.01), and the mean tumor mass was lighter (0.14 g vs 0.648 g, 0.635 g, P <0.01) The expression of KDR mRNA and protein in murine tumor tissue was significantly reduced. Conclusion Silencing KDR gene mediated by RNAi can significantly affect the tumor growth rate of PC-3 cells in nude mice, and KDR may be a new target for tumor therapy.