论文部分内容阅读
目的 探索丙型肝炎病毒 (HCV)基因型在山西省不同人群中的分布规律及流行的优势型。方法 用RT -PCR和型特异性引物逆转录巢式PCR法 ,对山西省 2 71例抗HCV阳性的丙型肝炎病人、原发性肝细胞癌患者、非肝癌癌症患者、性关系混乱者和性病患者、职业献血员、吸毒者及公共场所从业人员进行了HCVRNA的检测和基因分型。结果 2 71份抗HCV阳性标本中 ,HCVRNA检出率为 45 .45 %～ 89.6 6 % ,平均 6 7.5 2 %。以丙型肝炎病人、献血员和吸毒者的HCVRNA检出率较高 (76 .9%～ 89.7% ) ,χ2 =30 .44 ,P <0 .0 1。在 133份HCVRNA阳性血清中 ,仅检出了 10 8例 1b型、2a型和此两种基因型的混合感染者。未检出 1a型、2b型和 3a型。其中 1b型占 80 .0 0 % (88例 ) ,2a型占 11.81% (13例 ) ,混合型占 6 .36 % (7例 )。在肝癌患者和献血员中 ,仅检出 1b型的感染 ;非肝癌的其他癌症患者中 ,未发现混合感染。各基因型在各人群中的分布比例也有差别 ,丙型肝炎患者、非肝癌的其他癌症患者、吸毒者和从业人员的各基因型构成比较接近 ,均以 1b型为主。而性病患者和性关系混乱者中 1b型和 2a型感染者比例相等。结论 山西省HCV的基因以 1b型占优势
Objective To explore the distribution and prevalence of hepatitis C virus (HCV) genotypes in different populations in Shanxi Province. Methods Reverse transcriptase-PCR (RT-PCR) and type-specific primer reverse transcription-polymerase chain reaction (RT-PCR) were used to detect HCV infection in 2171 HCV-positive patients with hepatitis C, primary hepatocellular carcinoma, non-hepatocellular carcinoma, Patients with STDs, professional blood donors, drug addicts and practitioners in public places were tested for HCV RNA and genotyped. Results Among 721 anti-HCV positive samples, the detection rate of HCV RNA was 45.45% to 89.6% with an average of 6 7.52%. The detection rate of HCV RNA among hepatitis C patients, blood donors and drug addicts was high (76.9% -89.7%), χ2 = 30.44, P <0.01. Of 133 HCV RNA positive sera, only 108 co-infected patients of type 1b, type 2a and both genotypes were detected. Type 1a, 2b and 3a were not detected. Type 1b accounted for 80.0% (88 cases), type 2a accounted for 11.81% (13 cases) and mixed type accounted for 6.36% (7 cases). In liver cancer patients and blood donors, only type 1b infection was detected; in other cancer patients without liver cancer, no coinfections were found. The genotype distribution in each population is also different, hepatitis C patients, non-cancer of other cancer patients, drug users and practitioners of each genotype are relatively close, are mainly type 1b. In STD patients and confounders, the proportion of type 1b and 2a infections is the same. Conclusion The genotypes of HCV in Shanxi province are predominant in type 1b