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目的:探讨抗纤复方I号对酒精性肾损害时血小板源性生长因子-B(platelet-derivedgrowthfactor-B,PDGF-B)表达的影响。方法:按本科室创建的方法用乙醇及抗纤复方I号直接灌胃,于4周、8周、12周末处死大鼠,应用HE染色、透射电镜等观察其病理改变;用免疫组化方法检测中药组、酒精组、正常组肾组织PDGF-B的表达。结果:中药组同酒精组相比较,随着抗纤复方I号干预时间的延长,HE染色可见肾小球系膜增殖、间质局灶性炎性细胞浸润、肾小管上皮扁平化和小管扩张等病理改变出现延迟且明显减轻。免疫组化染色,中药组PDGF-B表达也明显低于酒精组(P<0.05)。结论:抗纤复方I号有效地防治酒精性肾损害,可能是促进了酒精的代谢,或干预了致肾间质纤维化的细胞因子PDGF的生成。
Objective: To investigate the effect of anti-fibrosis compound I on the expression of platelet-derived growth factor-B (PDGF-B) in alcoholic kidney injury. METHODS: Ethanol and anti-fibrosis compound I were directly intragastrically administered according to the method established in the undergraduate department. The rats were sacrificed at 4 weeks, 8 weeks, and 12 weeks. The pathological changes were observed with HE staining and transmission electron microscope; immunohistochemical method was used. The expression of PDGF-B in kidney tissue of Chinese medicine group, alcohol group and normal group was detected. RESULTS: Compared with the alcohol group, with the prolongation of anti-fibrosis compound I time, HE staining showed mesangial proliferation, interstitial focal inflammatory cell infiltration, flattening of tubular epithelium, and tubule expansion. Pathological changes such as delay and significantly reduced. Immunohistochemical staining showed that the expression of PDGF-B in the Chinese medicine group was significantly lower than that in the alcohol group (P<0.05). Conclusion: Anti-fibrosis compound I can effectively prevent and treat alcoholic kidney damage, which may promote the metabolism of alcohol, or interfere with the production of PDGF, a cytokine that causes renal interstitial fibrosis.