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目的探讨硫酸镁对胎儿生长受限(FGR)孕鼠胎盘组织半胱氨酸天冬氨酸蛋白酶3(caspase-3)表达的影响,及硫酸镁治疗FGR的机理。方法烟熏法构建FGR模型。实验对象分为对照组(10只)、治疗组(18只)、FGR组(10只)。治疗组中低剂量(硫酸镁300 mg／kg)治疗10只、高剂量(硫酸镁600 mg／kg)治疗8只,皮下注射给药。络合指示剂方法测孕鼠血清Mg2+(血镁)浓度。物理测量胎鼠的各项生理指标。链霉菌抗生物素蛋白-过氧化物酶连接(SP)法及RT-PCR法检测胎盘组织caspase-3的表达情况。结果(1)FGR组孕鼠血镁浓度为(0．55±0．03)mmol／L,高、低剂量治疗组孕鼠血镁浓度分别为(0．72±0．13)、(0．61±0．03)mmol／L,高、低剂量治疗组分别与FGR组比较,差异均有统计学意义(P<0．01)。(2)FGR组孕鼠胎盘重量为(0．63±0．05)g,其胎鼠体重为(2．95±0．46)g,高剂量治疗组分别为(0．80±0．16)、(3．58±0．10)g,两组分别比较,差异均有统计学意义(P<0．05、P<0．01)。(3)FGR组胎盘组织caspase-3 mRNA表达量为0．626±0．036,其蛋白表达量为199．5±4．7,高剂量治疗组分别为0．361±0．030、183．0±3．3,差异均有统计学意义(P< 0．05),低剂量治疗组caspase-3 mRNA表达量为0．525±0．029,与高剂量治疗组比较,差异也有统计学意义(P<0．05)。(4)孕鼠血镁浓度与胎鼠重量、胎盘组织caspase-3 mRNA及蛋白表达量有显著相关性(r=0．899,P=0．038;r=-0．747,P=0．033;r=-0．915,P=0．001)。结论硫酸镁能降低胎盘组织caspase-3 mRNA及蛋白表达,硫酸镁可能通过抑制胎盘caspase-3的表达,减少胎盘滋养细胞、血管内皮细胞等功能细胞的凋亡,从而改善FGR胎鼠的低体重现象。
Objective To investigate the effect of magnesium sulfate on the expression of caspase-3 in placenta of fetal rats with fetal growth restriction (FGR) and the mechanism of magnesium sulfate in the treatment of FGR. Methods The smoke method was used to construct FGR model. The subjects were divided into control group (n = 10), treatment group (n = 18) and FGR group (n = 10). The treatment group of low-dose (magnesium sulfate 300 mg / kg) treatment of 10, high-dose (magnesium sulfate 600 mg / kg) treatment of 8, subcutaneous injection. The complexation indicator method was used to measure the concentration of serum Mg2 + (blood magnesium) in pregnant rats. Physiological measurements of fetal physiological indicators. Streptavidin-peroxidase-linked (SP) method and RT-PCR method were used to detect the expression of caspase-3 in placenta. Results (1) The concentration of magnesium in the pregnant rats in FGR group was (0.55 ± 0.03) mmol / L, the levels of magnesium in pregnant rats in high and low dose groups were (0.72 ± 0.13) and .61 ± 0.03) mmol / L, high and low dose treatment groups compared with FGR group, the difference was statistically significant (P <0.01). (2) The weight of placenta of pregnant rats in FGR group was (0.63 ± 0.05) g, the body weight of fetus in FGR group was (2.95 ± 0.46) g, and the high dose group was (0.80 ± 0.46) g. 16) and (3.58 ± 0.10) g, respectively. There was significant difference between the two groups (P <0.05, P <0.01). (3) The expression of caspase-3 mRNA in placenta tissue of FGR group was 0.626 ± 0.036, the protein expression level was 199.5 ± 4.7, and the high-dose treatment group was 0.361 ± 0.030,183 .0 ± 3.3, the differences were statistically significant (P <0.05), low-dose treatment group caspase-3 mRNA expression was 0.525 ± 0.029, compared with the high-dose treatment group, the difference is also statistically Significance (P <0.05). (4) There was a significant correlation between serum concentration of magnesium in pregnant rats and fetal rat weight and expression of caspase-3 mRNA and protein in placenta (r = 0.899, P = 0.038; r = -0.747, P = .033; r = -0.915, P = 0.001). Conclusion Magnesium sulfate can reduce the expression of caspase-3 mRNA and protein in placenta. Magnesium sulfate may reduce the placental caspase-3 expression, reduce the apoptosis of functional cells such as placental trophoblast and vascular endothelial cells, phenomenon.