论文部分内容阅读
目的:探讨钙卫蛋白与新生儿坏死性小肠结肠炎(necrotizing enterocolitis，NEC)的相关性。方法:24只新生SD大鼠(SPF级)随机分为对照组(6只)及NEC组(18只)。对照组与母鼠同笼，自由摄食，NEC组采用人工喂养＋缺氧复氧＋冷刺激＋脂多糖灌胃连续3 d建立NEC模型。NEC组新生儿大鼠分别在造模后24、48、72 h各取6只空腹处死，取回盲部肠管进行组织病理学评分，并应用酶联免疫吸附法检测新生大鼠肠组织钙卫蛋白表达水平，实时定量聚合酶链反应法检测肠组织钙卫蛋白mRNA水平。结果:NEC组新生大鼠体重增长缓慢，造模后24、48、72 h肠组织病理学评分分别为2(2，3)、3(2，3)、4(3，4)分，均≥2分，NEC造模成功。NEC组造模后24、48、72 h新生大鼠肠组织钙卫蛋白表达水平分别为2.97(2.92，3.02)、3.64(3.36，3.93)、3.13(2.90，3.37)pg/ml，均高于对照组的1.89(1.85，1.92)pg/ml，并于造模后48 h达高峰，差异有统计学意义(n P<0.05)。NEC组造模后24、48、72 h新生大鼠肠组织钙卫蛋白mRNA水平分别为2.57(2.52，2.68)、3.23(3.18，3.36)、2.37(2.30，2.40)，均高于对照组的1.00(0.99，1.02)，并于造模后48 h达高峰，差异有统计学意义(n P<0.05)。n 结论:钙卫蛋白在新生大鼠NEC模型中表达明显升高，并且随着病程进展表现为先升高后下降的趋势。检测钙卫蛋白可能有助于新生儿NEC的诊断及病程判断。“,”Objective:To study the relationship between calprotectin and necrotizing enterocolitis (NEC) on neonatal rat model.Method:A total of 24 newborn Sprague-Dawley (SD) rats were randomly assigned into control group (6 rats) and NEC group (18 rats). The control group were fed freely by the mother rats in the same cage. NEC model was established following the protocol: artificial feeding, oxygen deprivation treatment, reoxygenation, cold stimulation and lipopolysaccharide gastric lavage for 3 days. After successful modeling, in the NEC group, 6 neonatal rats were sacrificed after fasting at 24, 48 and 72 h, respectively. The intestinal tract at ileocecal junction of the NEC group was studied histopathologically. The expression of calprotectin in the intestinal tissue was examined using enzyme-linked immunosorbent assay (ELISA) and the mRNA level of calprotectin was detected using real-time quantitative polymerase chain reaction (RT-PCR).Result:The pathology scores in NEC groups at 24, 48 and 72 h were 2(2, 3), 3(2, 3) and 4(3, 4). The calprotectin levels in intestinal tissue of NEC group were 2.97 (2.92, 3.02), 3.64 (3.36, 3.93) and 3.13 (2.90, 3.37) pg/ml at 24, 48 and 72 h after modeling, peaked at 48 h after modeling, and all higher than 1.89 (1.85, 1.92) pg/ml in control group (n P<0.05). The calprotectin mRNA levels in intestinal tissue of NEC group were 2.57 (2.52, 2.68), 3.23 (3.18, 3.36) and 2.37 (2.30, 2.40) at 24, 48 and 72 h after modeling, peaked at 48 h after modeling, and all higher than 1.00 (0.99, 1.02) in control group (n P<0.05).n Conclusion:The expression of calprotectin in neonatal rat NEC model is significantly increased. The expression of calprotectin first increases and then decreases as the disease progresses. These findings may contribute to a better diagnosis of NEC in newborns.