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采用3因素5水平二次旋转回归设计,以Hoagland营养液为基础,以pH、硝态氮(NO3--N)和铵态氮(NH4+-N)为处理因子进行水培试验,建立了多因子复合处理对甜菜(Beta vulgaris L.)品种甜研7号块根产量和含糖率影响的回归模型,并分析了各因子的主效应和互作效应。结果表明:在本试验条件下,影响甜菜块根产量和含糖率的单因子主效应大小均为NO3--N>pH>NH4+-N;pH在6.2~7.0范围内,对产量和含糖率的影响表现为正效应,在7.0~7.8之间,表现为负效应;NO3--N(0~16mmol.L-1)对产量表现正效应,而对含糖率则为负效应;在0~8.0mmol.L-1范围内,NH4+-N对产量影响为正效应,在8.0~16.0mmol.L-1之间为负效应,0~16mmol.L-1对含糖率影响为负效应。NO3--N与NH4+-N的互作对产量、pH与NO3--N的互作对含糖率的影响均达显著水平。模拟寻优结果表明,介质环境pH在6.5~7.5范围内,适当调节NO3--N和NH4+-N比例,可获得较高的甜菜块根产糖量,总N为16.0mmol.L-1、NO3--N:NH4+-N为16∶0时及N为19.2mmol.L-1、NO3--N∶NH4+-N为5∶1时产糖量最佳。
Based on Hoagland nutrient solution and hydroponic experiment with pH, nitrate nitrogen (NO3 - N) and ammonium nitrogen (NH4 + -N) as the treatment factors, a three - factorial and five - level quadratic regression model was established. Factor regression analysis was used to study the effects of compound treatment on the root yield and sugar content of sweet bean (Beta vulgaris L.) variety Yanyan 7 and the main and interaction effects of each factor were analyzed. The results showed that under the experimental conditions, the single factor main effects affecting the yield and sugar content of beet root tuber were NO3 - N> pH> NH4 + -N. The pH ranged from 6.2 to 7.0. (0 ~ 16mmol.L-1) showed a positive effect on the yield, but a negative effect on the sugar content; at 0 ~ 8.0mmol.L-1, the effect of NH4 + -N on the yield was a positive effect, with a negative effect between 8.0 ~ 16.0mmol.L-1 and a negative effect of 0 ~ 16mmol.L-1 on the sugar content . The effect of NO3 - N and NH4 + -N interaction on yield and pH and NO3 - N interaction have significant effects on the sugar content. The simulation results showed that the pH value of the medium was in the range of 6.5-7.5. The proper sugar content of sugar beet root tuber could be obtained by adjusting the ratio of NO3 - N and NH4 + -N, the total N was 16.0mmol.L-1, NO3 -N: NH4 + -N is 16:0 and N is 19.2mmol.L-1, NO3 - N: NH4 + -N is 5:1, the best sugar yield.