电镜样本常规制样技术,因受固定液渗透速率慢、电镜放大倍数高、组织观察范围有限等条件制约,要求每个包埋块组织大小仅为1 mm3。在日常工作中,常需要增加包埋块数以满足临床诊断的需要。我科通过近10年3万余例电镜标本制备总结出多组织块集合包埋制片法,既能大大减少工作量及成本,又不影响电镜观察。 Conventional sample preparation techniques for electron microscopy specimens are limited by the slow rate of penetration of the fixative solution, high electron microscope magnification, and limited scope for tissue observation, requiring that the size of each embedding block be 1 mm3. In daily work, often need to increase the number of embedded blocks to meet the needs of clinical diagnosis. Our department summarized more than 30,000 samples of electron microscopy in the past 10 years and summarized the multi-tissue block embedding preparation method, which can greatly reduce the workload and cost without affecting the electron microscope observation.