论文部分内容阅读
目的观察肝细胞癌患者癌组织中RASSF1A基因甲基化状态的临床意义,阐明WIF-1基因甲基化在肝细胞癌发生发展中的作用。方法 97例肝细胞癌患者手术切除癌组织蜡块标本为肝细胞癌组,随机留取26例手术切除肝细胞癌旁肝硬化组织蜡块标本为肝硬化组。两组标本经蜡块组织DNA提取、亚硫酸盐甲基化修饰,并采用甲基化特异性PCR法对抑癌基因RASSF1A和WIF-1进行目的片段的扩增,扩增产物通过电泳凝胶成像系统进行甲基化结果分析判定;根据患者术前实验室检测是否感染HBsAg,将肝细胞癌组细分为HBsAg阳性组及HBsAg阴性组,分析抑癌基因RASSF1A和WIF-1甲基化的意义。结果 RASSF1A基因甲基化率肝细胞癌组(67.01%)高于肝硬化组(42.31%),差异有统计学意义(P=0.021);WIF-1基因甲基化率肝细胞癌组(46.39%)高于肝硬化组(7.69%),差异有统计学意义(P<0.05);肝细胞癌组中抑癌基因RASSF1A和WIF-1基因甲基化表达均阳性为35.05%,肝硬化组为7.69%,差异有统计学意义(P=0.006)。HBsAg阳性肝细胞癌组RASSF1A基因的甲基化阳性率(72.84%)高于HBsAg阴性组(23.08%),差异有统计学意义(P=0.001);WIF-1基因在HBsAg阳性(44.44%)和阴性肝细胞癌组(53.84%)差异无统计学意义(P=0.528)。肝细胞癌患者RASSF1A和WIF-1基因的甲基化状态与生存率比较差异无统计学意义(P=0.735,P=0.229)。结论肝细胞癌患者癌组织较肝硬化组织RASSF1A,WIF-1基因甲基化率高,提示抑癌基因RASSF1A和WIF-1的甲基化在肝细胞癌的发生中起到一定作用。
Objective To observe the clinical significance of RASSF1A gene methylation status in hepatocellular carcinoma (HCC) patients and to elucidate the role of WIF-1 gene methylation in the occurrence and development of hepatocellular carcinoma. Methods Seventy-seven patients with hepatocellular carcinoma (HCC) were divided into two groups randomly: hepatocellular carcinoma (HCC) group and non-HCC group (HCC). Two groups of specimens were extracted with paraffin blocks and methylated with sulfite. The methylation-specific PCR method was used to amplify the target genes of tumor suppressor gene RASSF1A and WIF-1. The amplified products were electrophoresed through electrophoresis gel Imaging system for methylation analysis; according to the preoperative laboratory test whether the patient infected with HBsAg, the hepatocellular carcinoma group was subdivided into HBsAg-positive group and HBsAg-negative group, analysis of tumor suppressor genes RASSF1A and WIF-1 methylation significance. Results The methylation rate of RASSF1A gene in hepatocellular carcinoma (67.01%) was significantly higher than that in cirrhosis (42.31%) (P = 0.021) %) Was significantly higher than that of cirrhosis (7.69%) (P <0.05). The methylation of tumor suppressor gene RASSF1A and WIF-1 in hepatocellular carcinoma was 35.05% Was 7.69%, the difference was statistically significant (P = 0.006). The positive rate of methylation of RASSF1A gene in HBsAg positive hepatocellular carcinoma was 72.84%, which was significantly higher than that in HBsAg negative (23.08%) (P = 0.001). The positive rate of methylation of RASSF1A in HBsAg positive hepatocellular carcinoma was 44.44% And negative hepatocellular carcinoma (53.84%), the difference was not statistically significant (P = 0.528). The methylation status and survival rate of RASSF1A and WIF-1 genes in patients with hepatocellular carcinoma showed no significant difference (P = 0.735, P = 0.229). Conclusion The methylation rates of RASSF1A and WIF-1 gene in hepatocellular carcinoma are higher than those in cirrhosis, suggesting that the methylation of tumor suppressor genes RASSF1A and WIF-1 play a role in the development of hepatocellular carcinoma.