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通过所建立的检测石蜡包埋组织中人细小病毒B_(19)的套式PCR技术,检测了经石蜡包埋的66例自然流产组织、20例同期健康妇女人工流产组织及30例死胎儿肝脏组织、20例同期因患各种疾患死亡而尸检的新生儿肝组织。结果显示:自然流产组阳性率为30.3%而对照组阳性率为5%;死胎儿肝脏标本中有8例阳性而对照组中仅有1例阳性。套式PCR检测石蜡包埋组织中HPVB_(19)技术,为回顾性诊断流行病学调查,提供了一个有效的手段。
The established paraffin-embedded PCR detection of parvovirus B19 in paraffin-embedded tissue was used to detect 66 cases of spontaneous abortion, 20 cases of abortion and 30 cases of fetal death Organization, 20 cases of autopsy of neonatal liver tissue died of various diseases in the same period. The results showed that the positive rate was 30.3% in spontaneous abortion group and 5% in control group; 8 of the dead fetal liver samples were positive and only 1 of the control group was positive. Nested HPV detection of paraffin-embedded tissue HPVB_ (19) technology, for retrospective diagnosis of epidemiological investigations, provides an effective means.