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目的建立啤酒中玉米赤霉烯酮的高效液相色谱紫外检测法。方法啤酒中的玉米赤霉烯酮用乙酸乙酯提取,50℃水浴中氮气吹干,用乙腈溶解,过滤,以Zorbax SB-18为分离柱,用甲醇+0.020 mol/L乙酸铵(75+25)溶液为流动相,检测波长236nm。结果玉米赤霉烯酮在0μg/ml~10.0μg/ml范围内,线性关系良好(r=0.999 97);方法检出限5.0μg/kg。方法相对标准偏差(RSD)为2.3%~2.7%,加标回收率为86.0%~100.0%,平均回收率为93.6%。啤酒样品中玉米赤霉稀酮含量为51.6μg/kg~584.0μg/kg。结论本法具有简便、经济、灵敏、准确的特点,适宜在基层检测机构推广使用。
Objective To establish a high performance liquid chromatography (UV) detection method for zearalenone in beer. Methods The zearalenone in beer was extracted with ethyl acetate, dried in a nitrogen atmosphere at 50 ℃, dissolved in acetonitrile, filtered and separated on a Zorbax SB-18 column with methanol +0.020 mol / L ammonium acetate (75+ 25) solution as the mobile phase, detection wavelength of 236nm. Results The calibration curve of zearalenone in the range of 0μg / ml ~ 10.0μg / ml was linear (r = 0.999 97). The detection limit was 5.0μg / kg. The relative standard deviations (RSDs) were 2.3% -2.7%. The recoveries were in the range of 86.0% -100.0%. The average recoveries were 93.6%. The content of zeaxanthin in beer sample was 51.6μg / kg ~ 584.0μg / kg. Conclusion This law has the characteristics of simple, economical, sensitive and accurate, suitable for promotion and use in grass-roots testing agencies.