近年研究表明，乙型肝炎病毒（ＨＢＶ）感染者体内，可能存在ＨＢＶ前Ｃ区突变。我们根据ＨＢＶ前Ｃ区ＤＮＡ序列，建立了３′碱基特异性聚合酶链反应（３′ＢＳＰＣＲ）法，检测ＨＢＶ前Ｃ区第１８９６位核苷酸的突变，并检测了７２例ＨＢＶ感染者、１４例甲型肝炎患者的 Recent studies show that hepatitis B virus (HBV) infection in vivo, there may be mutations in pre-HBV C region. According to the HBV pre-C region DNA sequence, a 3 ’base-specific polymerase chain reaction (3’BSPCR) method was established to detect the mutation of the 1896th nucleotide in the pre-HBV C region and detected 72 HBV infection, 14 cases of hepatitis A patients