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目的探讨孕妇人群显性和隐匿性乙型肝炎病毒(HBV)感染及其HBV病毒株S基因的分子进化特征。方法 2014年6月1日—31日,在知情同意下,连续招募安徽省安庆市立医院产科初次住院活胎孕妇156人,收集其人口学特征和血清;对所有血清平行进行乙型肝炎病毒表面抗原(HBsAg)酶联免疫吸附法和电化学发光法检测、应用实时PCR定量测定HBV DNA、应用巢式PCR进行HBV S片段扩增、测序及进化分析。结果156名孕妇中,显性和隐匿性HBV感染率分别为10.3%(16/156)、8.6%(12/140);成功获得13株HBV S基因序列,其中显性7株,隐匿性6株;所有病毒株均为B基因型;仅1例隐匿性HBV株为ayw1血清型,其余12株均为adw2血清型。结论孕妇人群携带较高比例的显性和隐匿性HBV。
Objective To investigate the dominant and occult hepatitis B virus (HBV) infection in pregnant women population and the molecular evolution characteristics of S gene of HBV strain. Methods From June 1 to June 31, 2014, with the informed consent, 156 pregnant women with first-time hospitalized obstetric obstetrics in Anqing Municipal Hospital of Anhui Province were continuously enrolled in this study. Their demographic characteristics and serum were collected. All the serum samples were processed in parallel with the surface of hepatitis B virus Antigen (HBsAg) enzyme-linked immunosorbent assay and electrochemical luminescence detection, quantitative real-time PCR using HBV DNA, nested PCR for S segment of HBV amplification, sequencing and evolutionary analysis. Results Among 156 pregnant women, the positive and occult HBV infection rates were 10.3% (16/156) and 8.6% (12/140) respectively, and 13 HBV S gene sequences were successfully obtained, of which 7 were dominant and 6 were occult All strains were genotype B, only 1 occult HBV was serotype ayw1 and the remaining 12 were all adw2 serotypes. Conclusion Pregnant women carry a higher proportion of dominant and occult HBV.