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对聚偏氟乙烯(PVDF)中空纤维膜进行表面亲水化处理,再经活化、偶联制得了带有苯丙氨酸(Phe)的PVDF中空纤维亲和膜.制备过程中以光电子能谱(XPS)及红外光谱分析膜表面基团的变化,经亲水化处理及活化偶联后,膜表面的O和N含量分别从5.6％和0.6％增加到16.5％和3.0％,而F含量由38.3％降低为23.0％,将该亲和膜用于室温下血浆中γ-球蛋白的分离实验,在pH=7.4,离子强度I=0.18的缓冲液体系及进料质量浓度为1.0mg/mL条件下,该亲和膜对γ-球蛋白的吸附量可达到5.85mg/g膜;与市售γ-球蛋白相比,血浆中提取纯度可达83.9％。
Polyvinylidene fluoride (PVDF) hollow fiber membrane surface hydrophilization treatment, and then activated, coupled with a phenylalanine (Phe) PVDF hollow fiber affinity membrane preparation process to photoelectron spectroscopy (XPS) and infrared spectroscopy. The content of O and N on the membrane surface increased from 5.6% and 0.6% to 16.5% and 3.0% after hydrophilization and activation coupling, respectively. The content of F From 38.3% to 23.0%, the affinity membrane was used for the separation of γ-globulin from plasma at room temperature. The buffer solution with pH = 7.4, ionic strength I = 0.18 and the mass concentration of 1.0mg / mL, the affinity of the affinity membrane for γ-globulin up to 5.85mg / g membrane; compared with the commercially available γ-globulin, plasma purity of up to 83.9%.