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In this study,DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana,sequenced and analyzed by DNAsis. In addition,the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A,which was transformed into Agrobacterium tumefaciens. Furthermore,the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis,chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. Two positive transgenic plantlets were obtained in vitro. Based on PCR detection,DREB1A transcription factor was integrated into chrysanthemum genome,which laid the foundation for breeding new transgenic cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance,good quality and high yield.
In this study, DERB1A transcription factor and stress-induced promoter rd29A were isolated respectively and amplified from Arabidopsis thaliana, sequenced and analyzed by DNAsis. In addition, the stress-induced promoter rd29A was utilized to construct the plant expression vector of DERB1A, which was transformed into Agrobacterium tumefaciens. Furthermore, the transgenic regeneration system of fresh-cut chrysanthemum from callus to plantlets was established successfully. On this basis, chrysanthemum leaf-disc explants were genetically transformed with Agrobacterium-mediated method. . Based on PCR detection, DREB1A transcription factor was integrated into chrysanthemum genome, which laid the foundation for breeding new transgenic cultivars of fresh-cut chrysanthemum with high comprehensive stress resistance, good quality and high yield.