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Egbert等(1977)首先报告在将微孔乙酸纤维素材料压于结膜上后,眼结膜细胞可被取下、染色,并用光学显微镜检查。作者用这一技术的改良法检查100只眼,这些病人有或无眼结膜疾病。将4×6 mm乙酸过滤材料条用玻璃棒分别压于鼻侧和颞侧球结膜以及睑结膜上,然后轻轻提起。此时附着于过滤材料表面的上皮细胞和杯状细胞被取下呈细胞片,从1～2层细胞厚至全层厚。然后将其固定于95%乙醇内,用苏木素和过碘酸-Schiff试剂(PAS)染色,盖以浸油(作为透明剂)和盖玻片。另外也可将过滤材料连续在升级浓度乙醇中脱水,然后在二甲苯中固定,用封固胶永久封固。正常眼表面压印在光学显微镜下所见:上皮细胞为小的,紧密的,彼此粘连的,核-胞浆比例约1:1或1:2。杯状细胞很多呈一群,形如
Egbert et al. (1977) first reported that conjunctival cells can be removed, stained, and examined by light microscopy after the microporous cellulose acetate material is pressed against the conjunctiva. The authors examined 100 eyes with the improved technique of this technique, with or without conjunctival disease. The 4 × 6 mm strips of acetic acid filter material were pressed against the nasal and temporal bulbar conjunctiva and conjunctiva, respectively, with a glass rod and then gently lifted. At this time, the epithelial cells and goblet cells attached to the surface of the filter material were taken down to form cell sheets, and the thickness of the cells was increased from 1 to 2 layers to full thickness. It was then fixed in 95% ethanol, stained with hematoxylin and periodic acid-Schiff’s reagent (PAS), impregnated with oil (as a clearing agent) and coverslips. In addition, the filter material can also be continuously dehydrated in an upgrading concentration of ethanol, then fixed in xylene and permanently sealed with sealant. Normal ocular surface embossing is seen under light microscopy: epithelial cells are small, dense, adherent to each other with a nuclear-cytoplasmic ratio of about 1: 1 or 1: 2. Many goblet cells in a group, shaped like