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目的 :评价白介素17E(IL-17E)对过敏性哮喘患者嗜酸粒细胞的作用。方法 :以流式细胞检测的方法检测14例过敏性哮喘患者、15例过敏性非哮喘患者、14名无过敏正常人嗜酸粒细胞IL-17E及受体的表达,以酶联免疫吸附法(ELISA)测定血浆IL-17E的水平,并比较了不同组别间的差异。结果:过敏性哮喘患者血浆IL-17E水平[145(64~290)ng/L]显著高于无过敏正常人群[21(0~116)ng/L](P=0.012)。血浆IL-17E水平与第1秒用力呼气容积(FEV_1)占预计值百分比(FEV_1%)呈显著负相关(r=-0.443,P=0.003)。嗜酸粒细胞表面IL-17E特异性受体IL-17R B的表达在过敏性哮喘患者中[43.08%(33.96%~59.98%)]显著高于过敏性非哮喘患者[11.98%(6.33%~27.11%)](P=0.002)和无过敏正常人群[17.70%(10.97%~38.9%)](P=0.01),IL-17RA的表达也在过敏性哮喘患者中[97.94%(94.25%~98.74%)]显著高于过敏性非哮喘者[86.60%(27.48%~97.64%)](P=0.036)和无过敏正常人群[93.24%(42.38%~97.36%)](P=0.027)。嗜酸粒细胞表面IL-17RB的表达与IL-17RA的表达相关(r=0.809,P=0.001)。过敏性哮喘患者血浆IL-17E水平为145(64~290)ng/L,显著高于无过敏正常人群[21(0~116)ng/L](P=0.012),但与过敏性非哮喘者[80(42~156)ng/L]比较无显著差异(P=0.275)。结论:过敏性哮喘患者血浆IL-17E水平、嗜酸粒细胞表达其受体IL-17RA和IL-17RB增高,提示IL-17E在过敏性炎症中嗜酸粒细胞的活化中发挥作用。
AIM: To evaluate the effect of interleukin 17E (IL-17E) on eosinophils in allergic asthma. Methods: Flow cytometry was used to detect the expression of IL-17E and its receptor in 14 allergic asthmatic patients, 15 allergic non-asthmatic patients and 14 non-allergic normal subjects. Enzyme-linked immunosorbent assay (ELISA) to measure the level of plasma IL-17E, and to compare the differences between different groups. Results: The plasma levels of IL-17E in patients with allergic asthma [145 (64-290) ng / L] were significantly higher than those in non-allergic subjects [21 (0-116) ng / L] (P = .012). The level of plasma IL-17E was negatively correlated with FEV 1 (FEV 1%) (r = -0.443, P = 0.003). The expression of IL-17E-specific receptor IL-17R B on eosinophils was significantly higher in allergic asthmatic patients (43.08%, 33.96% -59.98%) than in allergic non-asthmatic patients (11.98%, 6.33% The expression of IL-17RA was also significantly higher in patients with allergic asthma (97.94% vs 94.9%, P = 0.002) and in non-allergic normal subjects (17.70%, 10.97% -38.9% 98.74%)] were significantly higher than those in allergic non-asthmatic patients (86.60% (27.48% -97.64%)] (P = 0.036) and those without allergy (93.24% (42.38% -97.36%)] (P = 0.027). The expression of IL-17RB on eosinophils was correlated with the expression of IL-17RA (r = 0.809, P = 0.001). The level of plasma IL-17E in patients with allergic asthma was 145 (64-290) ng / L, significantly higher than that in non-allergic subjects [21 (0-116) ng / L] (P = 0.012) [80 (42 ~ 156) ng / L] was no significant difference (P = 0.275). Conclusion: The level of IL-17E in plasma and the expression of IL-17RA and IL-17RB in eosinophils are increased in patients with allergic asthma, which suggests that IL-17E plays a role in the activation of eosinophils in allergic inflammation.