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目的：家蝇对拟除虫菊酯抗药性与细胞色素Ｐ４５０的ＣＹＰ６Ｄ１基因有关，采用分子生物学的特异性基因聚合酶链反应技术分析家蝇抗药性的发生、发展规律，探讨早期预测及防治策略。方法：提取雄性家蝇腹部ＤＮＡ作为反应模板，根据ＣＹＰ６Ｄ１基因序列设计特异性基因引物，进行３０个循环扩增，电泳分析扩增产物与生物测试结果比较。结果：Ｄ－Ｒ品系扩增阳性率１００％，敏感品系扩增阳性率为０，深圳、汕头、韶关、广州、茂名品系扩增阳性率分别为５９％、５６．３％、５４％、５５％及２５％。扩增阳性率与家蝇对拟除虫菊酯抗性倍数呈正相关（ｒ＝０．８３４４，Ｐ＜０．０５）。结论：聚合酶链反应技术应用于家蝇抗性发生、发展规律分析，早期诊断及抗药性发生机理研究有重要意义。
OBJECTIVE: To study the resistance and development of housefly to pyrethroids and the CYP6D1 gene of cytochrome P450, the gene-specific PCR technique was used to analyze the occurrence and development of housefly resistance and to explore the early prediction and control strategy. Methods: The abdomen DNA of male housefly was extracted as reaction template. According to the sequence of CYP6D1 gene, specific gene primers were designed and amplified for 30 cycles. The amplification products were compared with the biological test results by electrophoresis. Results: The positive rate of amplification of D-R strain was 100%, the positive rate of amplification of sensitive strain was 0, and the positive rates of amplification in Shenzhen, Shantou, Guangzhou, Maoming were 59%, 56.3%, 54%, 55 % And 25%. The positive rate of amplification was positively correlated with that of housefly (r = 0.8344, P <0.05). Conclusion: Polymerase chain reaction (PCR) has important implications for the development of housefly resistance, developmental regulation, early diagnosis and drug resistance mechanism.