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我们通过复制兔创伤性休克模型,用金属镉还原法检测一氧化氮(NO)代谢产物硝酸盐的变化,探讨外周血中NO浓度的改变在创伤性休克发生时的作用和意义。材料和方法1.复制创伤性休克模型雄性新西兰大耳白兔8只,重2.4±0.23kg,实验前禁食禁水12小时。3%戊巴比妥钠30mg/kg耳缘静脉麻醉,解剖显露双侧颈总动、静脉,一侧颈总动脉插管接U形血压计。稳定半小时后记录血压,创伤前颈动脉抽血测血气分析,颈静脉采血留取NO正常对照。采用一侧下肢股骨骨折法复制创伤性休克模型并将血压降至8kPa定为休克标准,于此时动脉穿刺测血气分析,分创伤后(Bp=11kPa)、休克时及休克后30和90分钟四个时间点颈静脉抽取
We traced the model of traumatic shock in rabbits by metal cadmium reduction method to detect the nitric oxide (NO) metabolite nitrate change, to explore the role of the change of NO concentration in peripheral blood in the occurrence of traumatic shock. MATERIALS AND METHODS 1. Duplicate traumatic shock model Eight male New Zealand white rabbits, weighing 2.4 ± 0.23kg, were banned for 12 hours before the experiment. 3% sodium pentobarbital 30mg / kg ear vein anesthesia, dissection revealed bilateral common carotid artery and vein, one side of the common carotid artery cannulation U-shaped sphygmomanometer. Blood pressure was recorded after half an hour of stabilization, blood gas analysis was performed on the pre-traumatic carotid blood sampling, and the normal control of NO was collected on the jugular vein. One side of the lower extremity femoral fracture method of traumatic shock model and the blood pressure was reduced to 8kPa as the shock standard, at this time arterial puncture blood gas analysis, after traumatic injury (Bp = 11kPa), shock and shock after 30 and 90 minutes Four time points jugular vein extraction