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原子力显微镜是一种新型纳米显微技术,具有高分辨率(亚纳米级)、无需对样品进行特殊处理、近生理环境下实时观测样品、原位液态环境下观测样品、可在分子水平上研究样品的物理化学特性等优点。近年来,原子力显微镜在蛋白质研究中取得了令人瞩目的进展。本文介绍了原子力显微镜的原理和操作模式,阐述了它在蛋白质成像、吸附、折叠与展开、组装、单分子识别以及其他与蛋白质有关研究中的应用,并展望了它在蛋白质研究中的前景。
Atomic force microscopy is a new type of nano-microscopy technique with high resolution (sub-nanometer scale) that eliminates the need for special handling of samples, real-time observation of samples in near-physiological environments, observation of samples in situ in liquid environments, and research at the molecular level Physicochemical properties of the sample and so on. In recent years, AFM has made remarkable progress in protein research. This paper introduces the principle and mode of operation of AFM, expounds its application in protein imaging, adsorption, folding and unfolding, assembly, single molecule recognition and other protein related research. It also foregrounds its prospect in protein research.