目的评估血液制品中甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝检测试剂盒的可行性并建立室内质量控制。方法选择市售生化试剂盒,自制室内质控品并制定质量控制范围,分别用分光光度计和生化仪器同时对血液制品中的甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝检测进行检测,甘油检测采用GPO-PAP酶比色法,上清蛋白采用邻笨三酚红比色法,血浆蛋白采用双缩脲比色法,游离Hb采用Trinder比色法,亚甲蓝采用萃取比色法。结果选出5种试剂盒并建立了室内质控范围;甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝2种仪器比对检测结果均相近(P>0.05);甘油、上清蛋白、血浆蛋白、游离Hb以及亚甲蓝用分光度计各检测48人份,其最高值分别为2.12 g/L、2.40 g/L、63.8 g/L、1.09 g/L、0.31μmol/L。结论血液质量控制项目可采用试剂盒可在分光光度计上进行检测,简单可行,建立室内质控,确保结果准确性。 Objective To assess the feasibility of glycerol, supernatant, plasma protein, free Hb and methylene blue detection kits in blood products and to establish in-house quality control. Methods Commercial biochemical kits were used to prepare home-made quality control products and to establish the quality control scope. The contents of glycerol, supernatant, plasma protein, free Hb, and methylene blue in blood products were measured simultaneously by spectrophotometer and biochemical analyzer Detection, glycerol detection using GPO-PAP enzyme colorimetric method, the supernatant protein using stupid triol red colorimetric method, the biochemical protein biuret colorimetric method, free Hb using Trinder colorimetric method, methylene blue using extraction ratio Color method. Results Five kinds of kits were selected and the range of indoor quality control was established. The results of glycerin, supernatant, plasma protein, free Hb and methylene blue were similar (P> 0.05) , Plasma protein, free Hb, and methylene blue were all detected by spectrophotometer. The highest values ​​were 2.12 g / L, 2.40 g / L, 63.8 g / L, 1.09 g / L and 0.31 μmol / L, respectively. Conclusion The blood quality control project can be detected on a spectrophotometer using a kit, which is simple and feasible to establish indoor quality control to ensure the accuracy of the results.